Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Mouse Monoclonal (IgG1) to Human FAK / Focal Adhesion Kinase
Human
Western blot, Immunoprecipitation
Agarose Conjugated
Details
Human FAK / Focal Adhesion Kinase
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Agarose
Purified
Unmodified
Applications
Western blot
Immunoprecipitation
Specificity and Use
GST fusion protein corresponding to residues 1-423 of human FAK.
aa1-423
Recognizes FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3).
Suitable for immunoprecipitation and affinity purification. Immunoprecipitation: 5-10 ug (10-20ul) of this lot of antibody-agarose immunoprecipitated FAK from a mouse 3T3/A31 RIPA cell lysate, as confirmed by subsequent immunoblot analysis using anti-FAK.
Packaging
Covalently linked to 200 ml of protein G agarose beads, provided as a 50% gel slurry suspended in PBS, 0.05% sodium azide for a total volume of 400ml. Liquid suspension.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development.