Human F9 / Factor IX

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity absorbed

Unmodified

Anticoagulation Factor IX

Human F9 / Factor IX. The defined antibody reactivity is restricted to Factor IX as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein and with hemophilia B plasma. Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

• Immunodiffusion
• Immunoelectrophoresis

This product is intended for use in precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FIX. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Plasma samples and all assay components must contain EDTA to stabilize the proteins. Normal adult plasma contains an average of 1 mg FIX per 100 ml. In a normal population this varies between 80% and 120% of the average. Newborn infants have a level of about 75% of the average adult level. Absence of FIX in plasma causes a bleeding diathesis very similar to hemophilia A in clinical manifestations and from the point of view of heredity. It is known as hemophilia B or Christmas disease. Different types have been described. The most common type demonstrates reduced FIX coagulant activity without measurable FIX relates antigen. Other types of reduced FIX antigen and coagulation activity, or reduced coagulant activity with normal antigen levels. Heterozygote carriers can be detected. Acquired depression of plasma FIX levels are seen in vitamin K deficiencies, in liver disease, or following therapy with vitamin K antagonists (oral coagulants).

Lyophilized antiserum

Use 1 ml of sterile Distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P00740 NCBI: NM_000133 NP_000124.1