Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
F9 / Factor IX antibody was raised against recombinant fragment corresponding to a region within amino acids 217 and 453 of Factor IX (SwissProt P00740). Percent identity by BLAST analysis: Human (100%); Monkey (96%); Dog, Pig (87%); Rabbit, Rat, Bovine (82%); Mouse (80%).
Human Factor IX
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
F9 Antibody, Christmas Disease Antibody, Coagulation factor IX Antibody, Factor IX Deficiency Antibody, FIX Antibody, HEMB Antibody, F9 p22 Antibody, Factor 9 Antibody, Factor IX Antibody, Factor IX F9 Antibody, FIX F9 Antibody, Haemophilia B Antibody, THPH8 Antibody, Christmas factor Antibody, Hemophilia B Antibody, PTC Antibody, Serine protease Antibody
F9 / Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII.