Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Human F8 / FVIII / Factor VIII
IHC, Western blot
Mouse Monoclonal (IgG2a) to Human F8 / FVIII / Factor VIII
Western blot, ELISA
Human F8 / FVIII / Factor VIII
Human (tested or 100% immunogen sequence identity)
Specificity and Use
F8 / FVIII / Factor VIII antibody was raised against human Factor VIII.
Recognizes the light chain of Factor VIII (83kD). Does not cross-react with von Willebrand Factor. Species cross-reactivity: Human. Others not tested.
Suitable for use in ELISA, Western Blot. When performing Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24:4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot.
No preservatives added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
F8 Antibody, AHF Antibody, Antihemophilic factor Antibody, Coagulation factor VIIIc Antibody, Coagulation factor VIII Antibody, DXS1253E Antibody, Factor VIII F8B Antibody, FVIII Antibody, HEMA Antibody, F8B Antibody, Factor VIII Antibody, Procoagulant component Antibody, F8C Antibody
F8 / FVIII / Factor VIII is coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex.