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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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F8 / FVIII / Factor VIII Antibody LS‑C23378
Factor VIII antibody LS-C23378 is an unconjugated mouse monoclonal antibody to human Factor VIII (F8 / FVIII). Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C23378-500
500 µl
$590

Popular F8 / FVIII / Factor VIII Products

Antibody
Species: Human
Applications: ELISA, Immunoelectrophoresis
Anti-F8 / Factor VIII antibody IHC of human skeletal muscle and capillaries. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2979 concentration 5 ug/ml.
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Anti-F8 / Factor VIII antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3124 concentration 10 ug/ml.
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Applications: IHC, IHC - Paraffin
F8 / Factor VIII antibody LS-C135701 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Rabbit, Sheep
Applications: Western blot
Western blot of. Factor VIII (S2194) pAb in extracts from HUVEC cells.
Species: Human, Mouse
Applications: Western blot

Product Description

Factor VIII antibody LS-C23378 is an unconjugated mouse monoclonal antibody to human Factor VIII (F8 / FVIII). Validated for ELISA and WB.
About F8 / FVIII / Factor VIII
F8 / FVIII / Factor VIII is coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. P00451 NM_000132 NP_000123.1

F8 Antibody, AHF Antibody, Antihemophilic factor Antibody, Coagulation factor VIIIc Antibody, Coagulation factor VIII Antibody, DXS1253E Antibody, Factor VIII F8B Antibody, FVIII Antibody, HEMA Antibody, F8B Antibody, Factor VIII Antibody, Procoagulant component Antibody, F8C Antibody


Specifications

Target
Human F8 / FVIII / Factor VIII
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (1:16000)
Immunogen
Human Factor VIII.
Specificity
Recognizes the light chain of Factor VIII (83kD). Does not cross-react with von Willebrand Factor. Species cross-reactivity: Human. Others not tested.
Usage
Suitable for use in ELISA, Western Blot. When performing Western Blot of plasma preparations, background, non-specific bands, caused by the serum proteins are common. More complex blocking solutions or higher salt concentrations can help remove non-specific bands. Consult reference 2 (Rotblat, et al., Biochemistry 24:4294-4300 (1985)) for additional tips with regard to detecting Factor VIII via Western Blot.
Presentation
No preservatives added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/17/2018

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