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Anti-F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11) IHC-plus™ LS-B2321

Note: This antibody replaces LS-C38064

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular F2RL1 / PAR2 Antibodies

Anti-F2RL1 / PAR2 Antibody (N-Terminus) IHC-plus™ LS-A252
Rabbit Polyclonal to Human F2RL1 / PAR2
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11) IHC-plus™ LS-B2321
Mouse Monoclonal [clone SAM11] (IgG2a) to Human F2RL1 / PAR2
Human
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone SAM11] (IgG2a) to Human F2RL1 / PAR2
Human
IHC - Paraffin, Immunofluorescence, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human F2RL1 / PAR2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [SAM11]
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

F2RL1 / PAR2 antibody was raised against synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.
N-Terminus
Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
Immunohistochemistry: LS-B2321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2321 was determined to be 5 ug/ml. Immunofluorescence: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About F2RL1 / PAR2

P55085 NM_005242 NP_005233.3

F2RL1 Antibody, PAR2 Antibody, Thrombin receptor-like 1 Antibody, Protease-activated receptor 2 Antibody, G-protein coupled receptor 11 Antibody, GPR11 Antibody, PAR-2 Antibody

Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection.

Immunohistochemistry

Immunohistochemistry
Anti-F2RL1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2321 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 2/27/2017

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