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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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F2R / Thrombin Receptor / PAR1 Antibody (N‑Terminus, clone ATAP2) IHC‑plus™ LS‑B2651
Note: This antibody replaces LS-C38062
PAR1 antibody LS-B2651 is an unconjugated mouse monoclonal antibody to human PAR1 (F2R / Thrombin Receptor). Validated for ELISA, Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2651-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

PAR1 antibody LS-B2651 is an unconjugated mouse monoclonal antibody to human PAR1 (F2R / Thrombin Receptor). Validated for ELISA, Flow, IF and IHC. Tested on 20 paraffin-embedded human tissues.
About F2R / Thrombin Receptor / PAR1
Thrombin Receptor is a Proteinase-Activated Receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. Thrombin induced RANTES expression through a PAR-1 mediated pathway influences inflammatory cell migration to the synovium in patients with rheumatoid arthritis. P25116 NM_001992 NP_001983.2

F2R Antibody, CF2R Antibody, Coagulation factor II receptor Antibody, PAR1 Antibody, PAR-1 Antibody, Thrombin receptor Antibody, TR Antibody, HTR Antibody, Protease-activated receptor 1 Antibody


Specifications

Target
Human F2R / Thrombin Receptor / PAR1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [ATAP2]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1 - 3 µg/ml)
  • Flow Cytometry (10 - 20 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Western blot
Immunogen
A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.
Epitope
N-Terminus
Specificity
Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.
Usage
Immunohistochemistry: LS-B2651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2651 was determined to be 10 ug/ml. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 ug/ml. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 ug/ml. ELISA: 0.1-1.0 ug/ml.
Presentation
PBS, pH 7.4, 0.1% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.

Immunohistochemistry

Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.

Immunohistochemistry

Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.

Immunohistochemistry

Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.
Anti-Thrombin Receptor antibody IHC of human placenta, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2651 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/17/2018

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