Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1,k) to Human F2 / Prothrombin / Thrombin
Western blot, ELISA
Human F2 / Prothrombin / Thrombin
Human (tested or 100% immunogen sequence identity)
Protein A/G purified
Specificity and Use
Thrombin isolated from human serum.
Recognizes human thrombin. Also reacts with hirudin, a direct inhibitor of thrombin. Does not cross-react with pro-thrombin. A strong reaction is seen in ELISA with thrombin directly coated onto the microtiter plate. Biotinylation of thrombin blocks antibody binding.
Suitable for use in ELISA and Western Blot. ELISA: 1:250.
PBS, pH 7.2, 15 mM sodium azide, 40% glycerol. No stabilizing proteins added.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.