Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, ICC, Immunofluorescence, Western blot
F12 / Factor XII
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:400)
Western blot (1:1000)
Specificity and Use
Synthetic peptide made to an internal portion of the human F12 protein (between residues 50-150)
In Western Blot, bands are seen ~67, 50, and 140kDa representing the full product, a clevage chain product, and a possible dimer. In ICC/IF, cytoplasmic staining was observed in NIH/3T3 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of human renal cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Antiserum, 0.05% sodium azide
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
F12 Antibody, Beta-factor XIIa part 2 Antibody, Beta-factor XIIa part 1 Antibody, Coagulation factor XII Antibody, F12 deficiency Antibody, Factor XII Antibody, Factor XII deficiency Antibody, HAE3 Antibody, HAEX Antibody, Hageman factor Antibody, HAF Antibody
F12 / Factor XII is coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain.