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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EYA2 Antibody (clone 2F8) LS‑C197173
EYA2 antibody LS-C197173 is an unconjugated mouse monoclonal antibody to human EYA2. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C197173-100
100 µg (0.5 mg/ml)
Unavailable

Popular EYA2 Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Western blot of EYA2 (arrow) using rabbit polyclonal EYA2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EYA2 gene.
Species: Human
Applications: Western blot
Detection of Human EYA2 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1000 (1 Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Detection of Human EYA2 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1000 (1 Detection: DAB.
Species: Human, Bovine, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

EYA2 antibody LS-C197173 is an unconjugated mouse monoclonal antibody to human EYA2. Validated for ELISA, IF and WB.
About EYA2
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. O00167 NM_005244 NP_005235.3

EYA2 Antibody, EAB1 Antibody, Eyes absent homolog 2 Antibody


Specifications

Target
Human EYA2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2F8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
EYA2 antibody was raised against eYA2 (NP_742108, aa164-271 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human EYA2
Usage
Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.
EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.

Western blot

Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.
EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.

Western blot

Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.
EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.

Western blot

Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.
EYA2 monoclonal antibody (M04), clone 2F8 Western Blot analysis of EYA2 expression in IMR-32.

Western blot

Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EYA2 expression in transfected 293T cell line by EYA2 monoclonal antibody (M04), clone 2F8.Lane 1: EYA2 transfected lysate(59.2 KDa).Lane 2: Non-transfected lysate.


Requested From: 
Date Requested: 6/25/2018

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