Rat EXOC4 / SEC8

Mouse

Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Trout (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

14G1

Unconjugated

Protein G purified

Unmodified

Recombinant rat rSec8 (his-tagged) expressed in E. coli.

Detects an ~110 kD protein, corresponding to the apparent molecular mass of rSec8 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout) guinea pig, hamster, monkey, pig, rabbit and sheep origins.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 µg/ml)
• Immunoprecipitation (12.5 µg/ml)

Immunohistochemistry: LS-B2987 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2987 was determined to be 5 ug/ml.

PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96A65 NCBI: NM_021807 NP_068579.3