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Anti-EXOC4 / SEC8 Antibody (clone 14G1) IHC-plus™ LS-B2987

Note: This antibody replaces LS-C63300

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular EXOC4 / SEC8 Antibodies

Anti-EXOC4 / SEC8 Antibody (clone 14G1) IHC-plus™ LS-B2987
Mouse Monoclonal [clone 14G1] (IgG1) to Rat EXOC4 / SEC8
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Trout
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-EXOC4 / SEC8 Antibody (aa71-120) LS-C117188
Rabbit Polyclonal to Human EXOC4 / SEC8
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Chicken
Western blot
Unconjugated
Western blot Image
Anti-EXOC4 / SEC8 Antibody (aa1-110, APC) LS-C245716
Mouse Monoclonal (IgG2a) to Human EXOC4 / SEC8
Human
Western blot, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 14G1] (IgG1) to Rat EXOC4 / SEC8
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Trout
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Rat EXOC4 / SEC8
Mouse
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Trout (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [14G1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation (12.5 µg/ml)

Specificity and Use

EXOC4 / SEC8 antibody was raised against recombinant rat rSec8 (his-tagged) expressed in E. coli.
Detects an ~110 kD protein, corresponding to the apparent molecular mass of rSec8 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, chicken, fish (rainbow trout) guinea pig, hamster, monkey, pig, rabbit and sheep origins.
Immunohistochemistry: LS-B2987 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2987 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, 0.1 mM PMSF, 50% glycerol.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About EXOC4 / SEC8

Q96A65 NM_021807 NP_068579.3

EXOC4 Antibody, Exocyst complex component Sec8 Antibody, Exocyst complex component 4 Antibody, KIAA1699 Antibody, Sec8p Antibody, SEC8-like 1 (S. cerevisiae) Antibody, SEC8 Antibody, SEC8-like 1 Antibody, SEC8L1 Antibody

EXOC4 / SEC8 is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery.

Immunohistochemistry

Immunohistochemistry
Anti-EXOC4 / SEC8 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2987 concentration 5 ug/ml.

Western blot

Western blot
Western analysis of rat brain tissue extract.

Requested From: United States
Date Requested: 12/8/2016

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