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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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EWSR1 / EWS Antibody (C‑Terminus) LS‑C110293
EWS antibody LS-C110293 is an unconjugated rabbit polyclonal antibody to EWS (EWSR1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C110293-50
50 µg
Unavailable

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Product Description

EWS antibody LS-C110293 is an unconjugated rabbit polyclonal antibody to EWS (EWSR1) from human, mouse, rat and other species. Validated for WB.
About EWSR1 / EWS
EWSR1 / EWS is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. Q01844 NM_005243 NP_005234.1

EWSR1 Antibody, EWS Antibody, RNA-binding protein EWS Antibody, BK984G1.4 Antibody, EWS oncogene Antibody


Specifications

Target
Human EWSR1 / EWS
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
EWSR1 / EWS antibody was raised against synthetic peptide from C-Terminus of human EWSR1 (Q01844, NP_005234). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus (100%); Zebra finch (92%); Xenopus (85%).
Blocking Peptide
LS-E13889 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110293. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human EWSR1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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