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Anti-EWSR1 / EWS Antibody (C-Terminus) LS-C110293

Catalog Size Price
LS-C110293-50 50 µg Unavailable

Related Products

49 EWSR1 / EWS Antibodies

Most Popular EWSR1 / EWS Antibodies

Anti-EWSR1 / EWS Antibody (clone 5C10) IHC-plus™ LS-B6082
Mouse Monoclonal [clone 5C10] (IgG2a,k) to Human EWSR1 / EWS
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-EWSR1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6082 concentration 5 ug/ml.
Anti-EWSR1 / EWS Antibody (Internal, clone EPR4647) IHC-plus™ LS-B7255
Rabbit Monoclonal [clone EPR4647] to Human EWSR1 / EWS
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Anti-EWSR1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7255 dilution 1:50.
Anti-EWSR1 / EWS Antibody (aa628-656) LS-C167853
Rabbit Polyclonal to Human EWSR1 / EWS
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Fluorescent confocal image of U251 cell stained with EWSR1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EWSR1 immunoreactivity is localized to Nucleus significantly.
Anti-EWSR1 / EWS Antibody (clone 5H7) LS-C204515
Mouse Monoclonal [clone 5H7] (IgG2b,k) to Human EWSR1 / EWS
Human
ICC, Immunofluorescence, Western blot
Unconjugated
An image of HeLa cell cultures stained with antibody EWSR1 / EWS antibody (green) and chicken antibody to vimentin (red). Blue is a DNA stain, and it is clear that in these cells, EWS is localized along with the DNA in the nucleus.
Anti-EWSR1 / EWS Antibody LS-C346054
Rabbit Polyclonal (IgG) to Human EWSR1 / EWS
Human, Mouse
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of HeLa cells.

100% Guaranteed
Rabbit Polyclonal to Human EWSR1 / EWS
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human EWSR1 / EWS
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

EWSR1 / EWS antibody was raised against synthetic peptide from C-Terminus of human EWSR1 (Q01844, NP_005234). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus (100%); Zebra finch (92%); Xenopus (85%).
LS-E13889 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110293. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human EWSR1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EWSR1 / EWS

Q01844 NM_005243 NP_005234.1

EWSR1 Antibody, EWS Antibody, RNA-binding protein EWS Antibody, BK984G1.4 Antibody, EWS oncogene Antibody

EWSR1 / EWS is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis.

Western blot

EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/26/2018

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