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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ESRRB / ERR Beta Antibody (aa38‑87) LS‑C79997
ERR Beta antibody LS-C79997 is an unconjugated rabbit polyclonal antibody to ERR Beta (ESRRB) from human, bat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C79997-50
50 µg
Unavailable

Popular ESRRB / ERR Beta Products

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Fetal Kidney Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: Western blot
Formalin-fixed and paraffin-embedded human brain tissue reacted with ESRRB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

ERR Beta antibody LS-C79997 is an unconjugated rabbit polyclonal antibody to ERR Beta (ESRRB) from human, bat, bovine and other species. Validated for WB.
About ESRRB / ERR Beta
Estrogen-related receptor beta (ERR beta) a NR3 Steroid Receptor, has been shown to affect early placental development. ERR beta also can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). ERR beta binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). O95718 NM_004452 NP_004443.3

ESRRB Antibody, DFNB35 Antibody, ERRb Antibody, ERRbeta-2 Antibody, ESRL2 Antibody, Estrogen receptor-like 2 Antibody, ERR-beta Antibody, ERRB2 Antibody, ERRbeta Antibody, NR3B2 Antibody, Nuclear receptor ERRB2 Antibody, Orphan nuclear receptor Antibody, ERR beta-2 Antibody, ERR2 Antibody, Estrogen-related receptor 2 Antibody, Estrogen-related receptor beta Antibody, Steroid hormone receptor ERR2 Antibody


Specifications

Target
Human ESRRB / ERR Beta
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa38-87 of human ESRRB (O95718, NP_004443). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%); Mouse, Rat, Elephant, Rabbit (92%); Lizard (91%); Opossum (85%).
Blocking Peptide
LS-E11276 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C79997. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa38-87
Specificity
Human ESRRB
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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