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Anti-ESRP2 / RBM35B Antibody (clone 22C5.H7.A6) LS-C153834


Wt. Vol. Conc. Price
100 µg - - Unavailable

Related Products

17 ESRP2 / RBM35B Antibodies

Most Popular ESRP2 / RBM35B Antibodies

Anti-ESRP2 / RBM35B Antibody (aa61-110) LS-C31389
Rabbit Polyclonal (IgG) to Mouse ESRP2 / RBM35B
Mouse, Human, Monkey, Rat, Dog, Rabbit
Western blot
Western blot Image
Anti-ESRP2 / RBM35B Antibody (aa36-85) LS-C101883
Rabbit Polyclonal (IgG) to Human ESRP2 / RBM35B
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Rabbit
Western blot
Western blot Image
Anti-ESRP2 / RBM35B Antibody (C-Terminus) LS-C144331
Rabbit Polyclonal to Human ESRP2 / RBM35B
Human, Mouse
Western blot
Immunofluorescence Image
Anti-ESRP2 / RBM35B Antibody (clone 22C5.H7.A6) LS-C153834
Mouse Monoclonal [clone 22C5.H7.A6] (IgG) to Mouse ESRP2 / RBM35B
Western blot, ELISA
Western blot Image
Anti-ESRP2 / RBM35B Antibody (N-Terminus) LS-C170178
Rabbit Polyclonal to Mouse ESRP2 / RBM35B
Mouse, Human
Western blot
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 22C5.H7.A6] (IgG) to Mouse ESRP2 / RBM35B
Western blot, ELISA


Mouse ESRP2 / RBM35B
Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [22C5.H7.A6]
Protein A purified


  • Western blot (1:1000)

Specificity and Use

ESRP2 / RBM35B antibody was raised against anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
This protein-A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kD in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300. [PubMed:20711167] [PMC:PMC2957203]

About ESRP2 / RBM35B

Q9H6T0 NM_024939 NP_079215.2

ESRP2 Antibody, RBM35B Antibody, RNA binding motif protein 35A Antibody, RNA-binding protein 35B Antibody, RNA-binding motif protein 35B Antibody, RNA binding motif protein 35B Antibody

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs.

Western blot

Western blot
Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes ug and 1 contain 5 ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.

Requested From: 
Date Requested: 4/25/2017

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