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Anti-ESRP1 / RBM35A Antibody (clone 27H12) LS-C154126


Wt. Vol. Conc. Price
100 µg - - Unavailable

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18 ESRP1 / RBM35A Antibodies

Most Popular ESRP1 / RBM35A Antibodies

Anti-ESRP1 / RBM35A Antibody (aa161-210) LS-C82450
Rabbit Polyclonal (IgG) to Human ESRP1 / RBM35A
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Pig, Rabbit, Chicken
Western blot
Western blot Image
Anti-ESRP1 / RBM35A Antibody (N-Terminus) IHC-plus™ LS-B7036
Rabbit Polyclonal to Human ESRP1 / RBM35A
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-ESRP1 / RBM35A Antibody (aa182-211) LS-C167832
Rabbit Polyclonal to Human ESRP1 / RBM35A
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Anti-ESRP1 / RBM35A Antibody (aa189-219, APC) LS-C235073
Rabbit Polyclonal (IgG) to Human ESRP1 / RBM35A
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Anti-ESRP1 / RBM35A Antibody (aa189-219, Biotin) LS-C235074
Rabbit Polyclonal (IgG) to Human ESRP1 / RBM35A
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100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 27H12] (IgG) to Mouse ESRP1 / RBM35A
Western blot, ELISA


Mouse ESRP1 / RBM35A
Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [27H12]
Protein A purified


  • Western blot (1:1000)

Specificity and Use

ESRP1 / RBM35A antibody was raised against anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kD in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP. The EMBO journal. 2010 29:3286-300. [PubMed:20711167] [PMC:PMC2957203]

About ESRP1 / RBM35A

Q6NXG1 NM_017697 NP_060167.2

ESRP1 Antibody, RBM35A Antibody, RMB35A Antibody, RNA binding motif protein 35A Antibody, RNA-binding motif protein 35A Antibody, RNA-binding protein 35A Antibody

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs.

Western blot

Western blot
Anti-ESRP-1 Antibody - Western Blot. Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Requested From: 
Date Requested: 4/25/2017

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