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Anti-ESPL1 / Separase Antibody (phospho-Ser801) LS-C199560

Catalog Size Price
LS-C199560-50 50 µl Unavailable
LS-C199560-100 100 µl Unavailable

Most Popular ESPL1 / Separase Antibodies

Anti-ESPL1 / Separase Antibody (clone 6H6) IHC-plus™ LS-B6199
Mouse Monoclonal [clone 6H6] (IgG2a,k) to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6199 concentration 3 ug/ml.
Anti-ESPL1 / Separase Antibody (aa767-816) IHC-plus™ LS-B8366
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8366 dilution 5 ug/ml.
Anti-ESPL1 / Separase Antibody (Ala795) LS-C176573
Rabbit Polyclonal to Human ESPL1 / Separase
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Anti-ESPL1 / Separase Antibody (phospho-Ser801) LS-C354138
Rabbit Polyclonal to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-ESPL1 / Separase Antibody (N-Terminus) IHC-plus™ LS-B13240
Rabbit Polyclonal to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

100% Guaranteed
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human ESPL1 / Separase
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)

Specificity and Use

Synthetic peptide derived from human SEPARASE around the phosphorylation site of Ser801.
pSer801
SEPARASE (Phospho-Ser801) Antibody detects endogenous levels of SEPARASE only when phosphorylated at serine801.

Packaging

PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C.
For research use only.

About ESPL1 / Separase

Q14674 NM_012291 NP_036423.4

ESPL1 Antibody, ESP1 Antibody, Extra spindle poles like 1 Antibody, KIAA0165 Antibody, Caspase-like protein ESPL1 Antibody, SEPA Antibody, Separase Antibody, Separin Antibody

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex.

Immunohistochemistry

IHC of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right was incubated with synthetic peptide.

Requested From: 
Date Requested: 5/27/2018

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