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Anti-ESPL1 / Separase Antibody (phospho-Ser1126) LS-C96968

Catalog Size Price
LS-C96968-400 400 µl Unavailable

Most Popular ESPL1 / Separase Antibodies

Anti-ESPL1 / Separase Antibody (clone 6H6) IHC-plus™ LS-B6199
Mouse Monoclonal [clone 6H6] (IgG2a,k) to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6199 concentration 3 ug/ml.
Anti-ESPL1 / Separase Antibody (aa767-816) IHC-plus™ LS-B8366
Rabbit Polyclonal (IgG) to Human ESPL1 / Separase
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8366 dilution 5 ug/ml.
Anti-ESPL1 / Separase Antibody (Ala795) LS-C176573
Rabbit Polyclonal to Human ESPL1 / Separase
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
Anti-ESPL1 / Separase Antibody (phospho-Ser801) LS-C354138
Rabbit Polyclonal to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-ESPL1 / Separase Antibody (N-Terminus) IHC-plus™ LS-B13240
Rabbit Polyclonal to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

100% Guaranteed
Rabbit Polyclonal to Human ESPL1 / Separase
Human
IHC, IHC - Paraffin, Dot Blot
Unconjugated

Details

Human ESPL1 / Separase
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Dot Blot (1:500)

Specificity and Use

LS-E7316 - Lyophilized - 100 µg - $230.00
Immunizing peptide used to generate LS-C96968. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
pSer1126
This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ESPL1 / Separase

Q14674 NM_012291 NP_036423.4

ESPL1 Antibody, ESP1 Antibody, Extra spindle poles like 1 Antibody, KIAA0165 Antibody, Caspase-like protein ESPL1 Antibody, SEPA Antibody, Separase Antibody, Separin Antibody

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Dot Blot

Dot blot of anti-hSeparase-S801 Phospho-specific antibody on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Dot blot of anti-hSeparase-S801 Phospho-specific antibody on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.

Requested From: 
Date Requested: 5/25/2018

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