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ESET / SETDB1

SET domain, bifurcated 1

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

Gene Name: SET domain, bifurcated 1
Synonyms: SETDB1, H3-K9-HMTase4, ESET, KMT1E, H3-K9-HMTase 4, KG1T, SET domain, bifurcated 1, SET domain bifurcated 1, KIAA0067, Lysine N-methyltransferase 1E
Target Sequences: NM_012432 NP_036564.3 Q15047

Publications (4)

1
Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. Harte PJ, Wu W, Carrasquillo MM, Matera AG. Cytogenetics and cell genetics. 1999 84:83-6. [PubMed:10343109] Related Antibodies: LS-B720.
2
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ. Genes & development. 2002 16:919-32. [PubMed:11959841] [PMC:PMC152359] Related Antibodies: LS-B720.
3
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y. Molecular cell. 2003 12:475-87. [PubMed:14536086] Related Antibodies: LS-B720.
4
Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Sarraf SA, Stancheva I. Molecular cell. 2004 15:595-605. [PubMed:15327775] Related Antibodies: LS-B720.
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☰ Filters
Antibodies (27)
Proteins (9)
Over-Expression Lysate (4)
Primary (27)
Recombinant (5)
ESET / SETDB1 (36)
Human (27)
Mouse (10)
Rat (6)
Chimpanzee (3)
Monkey (2)
Non-Human Primate (2)
Orangutan (3)
IHC (8)
IHC-P (2)
WB (26)
ELISA (14)
ICC (1)
IF (3)
Peptide-ELISA (1)
rabbit (21)
mouse (6)
Product Group
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IgG (14)
IgG1 (6)
monoclonal mc (6)
polyclonal pc (21)
5H6A12 (2)
5H6D4 (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (19)
aa1193-1225 (7)
N-Terminus (4)
aa1-397 (1)
aa1125-1175 (1)
No (35)
Yes (1)
GST, N-terminus (3)
Myc-DDK (Flag) (6)
Human (7)
293T Cells (2)
E. coli (1)
HEK 293 Cells (4)
Wheat Germ Extract (2)
Purified (1)
ESET / SETDB1 Antibody - Anti-SETDB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$515
ESET / SETDB1 Antibody - Immunofluorescence of LOVO cells using SETDB1 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ESET / SETDB1 Mouse anti-Human Monoclonal (5H6A12) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$435
ESET / SETDB1 Antibody - SETDB1 Antibody in Immunofluorescence (IF)
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ESET / SETDB1 Mouse anti-Human Monoclonal (5H6A12) Antibody
Mouse, Human, Non-Human Primate
ICC, IF, WB
Unconjugated
100 µl/$541
ESET / SETDB1 Antibody - Western blot analysis of ESET expression in MCF7 (A); HeLa (B); A549 (C); HepG2 (D) whole cell lysates.
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
ESET / SETDB1 Antibody - Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1-397) Antibody
Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
ESET / SETDB1 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
GST, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ESET / SETDB1 Antibody - Immunofluorescence (IF) analysis of LOVO cells using ESET Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ESET / SETDB1 Mouse anti-Human Monoclonal Antibody
Mouse, Human, Monkey
ELISA, IF, WB
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
ESET / SETDB1 Antibody - Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue  using SETDB1 Polyclonal Antibody at dilution of 1:60(×200)
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ESET / SETDB1 Antibody - Western blot using SETDB1 mouse monoclonal antibody against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate.
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ESET / SETDB1 Mouse anti-Human Monoclonal (5H6D4) Antibody
Human
ELISA, WB
Unconjugated
100 µl/$424
ESET / SETDB1 Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$287; 50 µl/$341; 100 µl/$431; 200 µl/$539
ESET / SETDB1 Antibody - Western Blot (WB) analysis using ESET Monoclonal Antibody against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate.
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ESET / SETDB1 Mouse anti-Human Monoclonal Antibody
Mouse, Human, Monkey
ELISA, WB
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
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ESET / SETDB1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ESET / SETDB1 Antibody - SETDB1 Antibody in Western Blot (WB)
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ESET / SETDB1 Mouse anti-Human Monoclonal (5H6D4) Antibody
Mouse, Human, Non-Human Primate
WB
Unconjugated
100 µl/$518
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1125-1175) Antibody
Human
WB
Unconjugated
10 µg/$295; 100 µl/$514
ESET / SETDB1 Antibody - Western blot of Human HT1080 Whole cell . SETDB1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ESET / SETDB1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
ESET / SETDB1 Antibody - Western blot of anti-SETDB1 Pabin Ramos cell line lysate (35 ug/lane). SETDB1(arrow) was detected using the purified antibody.
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ESET / SETDB1 Rabbit anti-Human Polyclonal (aa1193-1225) Antibody
Human
WB
Unconjugated
400 µl/$393
Viewing 1-25 of 36 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).