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| Catalog Number | Size | Price |
|---|---|---|
| LS-B680-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human ERN1 / IRE1 Antibody (aa700‑800, IHC, WB) LS‑B680
IHC‑plus™ Polyclonal Rabbit anti‑Human ERN1 / IRE1 Antibody (aa700‑800, IHC, WB) LS‑B680
Note: This antibody replaces LS-C2796, LS-C71084
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
IRE1 antibody LS-B680 is an unconjugated rabbit polyclonal antibody to IRE1 (ERN1) (aa700-800) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ERN1 / IRE1
Synonyms
ERN1 | ER to nucleus signalling 1 | Inositol-requiring enzyme 1 | Inositol-requiring protein 1 | Ire1-alpha | IRE1a | HIRE1p | IRE1 | Inositol-requiring 1 | IRE1P
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Non-Reactivity
Primate
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide within the human IRE-1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Epitope
aa700-800
Specificity
IRE-1 alpha.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B680 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B680 was determined to be 5 ug/ml.Also tested in IHC with rat renal sections (paraffin) at 5 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ERN1 / IRE1
Validation
Anti-ERN1 / IRE1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ERN1 / IRE1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ ERN1 / IRE1 Antibody (aa700-800) for IHC, WB/Western LS-B680 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Monkey, Rabbit
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
ICC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
