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Anti-ERN1 / IRE1 Antibody (aa700-800) IHC-plus™ LS-B680

Note: This antibody replaces LS-C2796, LS-C71084

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ERN1 / IRE1 Antibodies

Anti-ERN1 / IRE1 Antibody (C-Terminus) IHC-plus™ LS-B8029
Rabbit Polyclonal to Human ERN1 / IRE1
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ERN1 / IRE1 Antibody (aa700-800) IHC-plus™ LS-B680
Rabbit Polyclonal to Human ERN1 / IRE1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ERN1 / IRE1
Human, Mouse, Rat
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ERN1 / IRE1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Primate
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • (applications tested for the base form of this product only)

Specificity and Use

ERN1 / IRE1 antibody was raised against a synthetic peptide within the human IRE-1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
aa700-800
IRE-1 alpha.
Immunohistochemistry: LS-B680 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B680 was determined to be 5 ug/ml.Also tested in IHC with rat renal sections (paraffin) at 5 ug/ml.

Packaging

Tris-glycine, 150mM NaCl, 0.05% Sodium Azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About ERN1 / IRE1

O75460 NM_001433 NP_001424.3

ERN1 Antibody, ER to nucleus signalling 1 Antibody, Inositol-requiring enzyme 1 Antibody, Inositol-requiring protein 1 Antibody, Ire1-alpha Antibody, IRE1a Antibody, HIRE1p Antibody, IRE1 Antibody, Inositol-requiring 1 Antibody, IRE1P Antibody

The endoribonuclease IRE1/ERN1 encodes the ER-to-nucleus signaling 1 protein, a human homolog of the yeast Ire1 gene product. In S. cerevisiae, as well as in human, this ER transmembrane IRE serine/threonine protein kinase monitors the status of unfolded proteins inside the ER lumen in response to stress. Activation of ERN1 leads to oligomerization, autophosphorylation, and proteolytic cleavage.

Immunohistochemistry

Immunohistochemistry
Anti-ERN1 / IRE1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B680 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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