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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERN1 / IRE1 Antibody (aa200‑300, DY488) LS‑C141646
IRE1 antibody LS-C141646 is a DY488-conjugated rabbit polyclonal antibody to IRE1 (ERN1) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C141646-100
100 µl
Unavailable

Popular ERN1 / IRE1 Products

Immunocytochemistry of IRE1p in A20 cells with IRE1p antibody at 1 µg/mL.
Species: Human, Mouse, Rat
Applications: ICC, Western blot
Anti-ERN1 / IRE1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B680 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody (0.03 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Human, Monkey, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-ERN1 / IRE1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4154 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-ERN1 / IRE1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8029 dilution 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot

Product Description

IRE1 antibody LS-C141646 is a DY488-conjugated rabbit polyclonal antibody to IRE1 (ERN1) from human and mouse. Validated for IHC and WB.
About ERN1 / IRE1
The endoribonuclease IRE1/ERN1 encodes the ER-to-nucleus signaling 1 protein, a human homolog of the yeast Ire1 gene product. In S. cerevisiae, as well as in human, this ER transmembrane IRE serine/threonine protein kinase monitors the status of unfolded proteins inside the ER lumen in response to stress. Activation of ERN1 leads to oligomerization, autophosphorylation, and proteolytic cleavage. O75460 NM_001433 NP_001424.3

ERN1 Antibody, ER to nucleus signalling 1 Antibody, Inositol-requiring enzyme 1 Antibody, Inositol-requiring protein 1 Antibody, Ire1-alpha Antibody, IRE1a Antibody, HIRE1p Antibody, IRE1 Antibody, Inositol-requiring 1 Antibody, IRE1P Antibody


Specifications

Target
Human ERN1 / IRE1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ERN1 / IRE1 antibody was raised against a synthetic peptide within the human IRE1 alpha protein (within residues 200-300). [Swiss-Prot #O75460]
Epitope
aa200-300
Specificity
IRE1 alpha.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry - Paraffin

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of IRE-1 alpha. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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