Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
Specificity and Use
ERK1 + ERK2 antibody was raised against synthetic peptide corresponding to aa174-183 (CTGFLTE[pY]-VAT) of human MAP Kinase.
Specific for phosphorylated Erk1 and Erk2 (42-44kD). Species cross-reactivity: Human, mouse and rat. Species sequence homology: rat, mouse and bovine MAP kinase-100%.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system was used. Immunoprecipitation: 4 ug immunoprecipitates pMAPK from a 500 ug of EGF-stimulated A431 RIPA lysate.