Rat ERK1 + ERK2

Rabbit

Rat, Human, Mouse, Hamster (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.

Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.

• IHC
• IHC - Paraffin
• ICC
• Immunofluorescence
• Western blot (1:1000)
• Immunoprecipitation (1:50)
• Flow Cytometry (1:25)
• ELISA

Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25.

10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.