Rat, Human, Mouse, Hamster
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:25)
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ERK1 + ERK2 antibody was raised against synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.
Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.