Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1 - 2 µg/ml)
ERCC1 antibody was raised against human, recombinant full length ERCC1 protein (MW 33-36kD). Cellular Localization: Nuclear.
Recognizes human ERCC1 (Excision Repair Cross Complement Group 1). Species cross-reactivity: rat.
Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 2 ug/mg protein lysate using Protein A (Denatured verified). Western Blot: 1-2 ug/ml for 2hrs at RT. Positive control: A431 or HeLa cells. Tonsil.
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
ERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion.