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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERCC1 Antibody (clone 2D8) LS‑C115212
ERCC1 antibody LS-C115212 is an unconjugated mouse monoclonal antibody to ERCC1. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C115212-100
100 µl
Unavailable

Popular ERCC1 Products

Immunohistochemical staining using ERCC1 antibody on formalin fixed, paraffin embedded human tonsil tissues.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-ERCC1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3956 concentration 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical analysis of ERCC1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog
Applications: IHC, IHC - Paraffin, Western blot

Product Description

ERCC1 antibody LS-C115212 is an unconjugated mouse monoclonal antibody to ERCC1. Validated for Flow, IF and WB.
About ERCC1
ERCC1 functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. P07992 NP_973730.1

ERCC1 Antibody, COFS4 Antibody, UV20 Antibody, RAD10 Antibody


Specifications

Target
Human ERCC1
Host
Mouse
Clonality
IgG2b Monoclonal [2D8]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:3000)
  • Flow Cytometry (1:100)
Immunogen
ERCC1 antibody was raised against full length human recombinant protein of human ERCC1 (NP_001974) produced in HEK293T cell
Specificity
Human ERCC1
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.11% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-ERCC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ERCC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ERCC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ERCC1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Anti-ERCC1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/20/2018

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