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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERBB4 / HER4 Antibody (phospho‑Tyr1284) LS‑C199055
HER4 antibody LS-C199055 is an unconjugated rabbit polyclonal antibody to HER4 (ERBB4) from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199055-50
50 µl
Unavailable
LS-C199055-100
100 µl
Unavailable

Popular ERBB4 / HER4 Products

Anti-ERBB4 antibody LS-A2847 IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
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Species: Human, Mouse, Rat
Applications: Immunoprecipitation, Flow Cytometry
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Species: Human
Applications: Flow Cytometry
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Species: Human
Applications: Flow Cytometry, Inhibition
IHC of paraffin-embedded human brain tissue, using HER4 (Ab-1284) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

HER4 antibody LS-C199055 is an unconjugated rabbit polyclonal antibody to HER4 (ERBB4) from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
About ERBB4 / HER4
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Q15303 NM_005235 NP_005226.1

ERBB4 Antibody, ERBB 4 Antibody, HER4 Antibody, p180 erb4 Antibody, p180erbB4 Antibody


Specifications

Target
Human ERBB4 / HER4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ERBB4 / HER4 antibody was raised against synthetic peptide derived from human HER4 around the phosphorylation site of Tyr1284.
Epitope
pTyr1284
Specificity
HER4 (Phospho-Tyr1284) Antibody detects endogenous levels of HER4 only when phosphorylated at tyrosine1284.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Immunofluorescence

Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with EGF 200nM 5', using HER4 (Phospho-Tyr1284) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HUVEC cells treated with EGF 200ng/ml 30', using HER4 (Phospho-Tyr1284) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/25/2018

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