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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ERBB2 / HER2 Antibody (aa1243-1255) IHC-plus™ LS-B2133

Note: This antibody replaces LS-C23217, LS-C92211, LS-C41661
HER2 antibody LS-B2133 is an unconjugated rabbit polyclonal antibody to HER2 (ERBB2) from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2133-50
50 µg (1 mg/ml)
$425
Description
HER2 antibody LS-B2133 is an unconjugated rabbit polyclonal antibody to HER2 (ERBB2) from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ERBB2 / HER2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ERBB2 / HER2 antibody was raised against a KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%); Elephant, Bat, Bovine (92%); Opossum (85%).
Epitope
aa1243-1255
Specificity
Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Usage
Immunohistochemistry: LS-B2133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2133 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 ug immunoprecipitated c-erbB-2 from 500 ug of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ERBB2 / HER2
P04626 NM_004448 NP_004439.2

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Immunohistochemistry

Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Requested From: United States
Date Requested: 4/23/2019
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