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Anti-ER Alpha / Estrogen Receptor Antibody (Ser167) IHC-plus™ LS-B1470

Note: This antibody replaces LS-C34304, LS-C65720, LS-C90439, LS-C118112, LS-C51211

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ER Alpha / Estrogen Receptor Antibodies

Anti-ER Alpha / Estrogen Receptor Antibody (clone AER314, Biotin) LS-C87826
Mouse Monoclonal [clone AER314] (IgG1,l) to Bovine ER Alpha / Estrogen Receptor
Bovine, Human, Mouse, Rat, Rabbit
Immunoprecipitation, Gel shift
Biotin Conjugated
Anti-ER Alpha / Estrogen Receptor Antibody (clone 1D5, Azide-free) LS-C88115
Mouse Monoclonal [clone 1D5] (IgG1,k) to Human ER Alpha / Estrogen Receptor
Human, Monkey, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-ER Alpha / Estrogen Receptor Antibody (clone SP1) LS-C88824
Rabbit Monoclonal [clone SP1] (IgG) to Human ER Alpha / Estrogen Receptor
Human, Hamster
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human ER Alpha / Estrogen Receptor
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human ER Alpha / Estrogen Receptor
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)

Specificity and Use

ER Alpha / Estrogen Receptor antibody was raised against synthetic peptide from human ESR1 / Estrogen receptor.
Ser167
Amino acids surrounding Ser 167 of human ESR1
Immunohistochemistry: LS-B1470 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1470 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

1
ER beta -mediated estradiol enhances epithelial mesenchymal transition of lung adenocarcinoma through increasing transcription of midkine. Zhao G, Nie Y, Lv M, He L, Wang T, Hou Y. Molecular endocrinology (Baltimore, Md.). 2012 Aug;26:1304-15. [Full Text Article] [PubMed:22669742]
2
Dienogest increases the progesterone receptor isoform B/A ratio in patients with ovarian endometriosis. Hayashi A, Tanabe A, Kawabe S, Hayashi M, Yuguchi H, Yamashita Y, Okuda K, Ohmichi M. Journal of ovarian research. 2012 5:31. [Full Text Article] [PubMed:23113924] [PMC:PMC3541078]

About ER Alpha / Estrogen Receptor

P03372 NM_000125 NP_000116.2

ESR1 Antibody, Era Antibody, ERalpha Antibody, Estrogen receptor Antibody, Estradiol receptor Antibody, ER-alpha Antibody, Estrogen receptor 1 Antibody, NR3A1 Antibody, ER Antibody, ESR Antibody, ESRA Antibody, Estrogen receptor alpha Antibody

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.

Immunohistochemistry

Immunohistochemistry
Anti-Estrogen Receptor antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1470 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Ab-167) antibody.

Western blot

Western blot
Western blot of extracts from MCF cells using Estrogen Receptor-a(Ab-167) antibody and the same antibody preincubated with blocking peptide.
 

Requested From: United States
Date Requested: 12/4/2016

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