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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ER Alpha / Estrogen Receptor Antibody (clone 6F11) LS‑C11577
Note: This antibody replaces LS-C43920
Estrogen Receptor antibody LS-C11577 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha). Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C11577-1000
1000 µl
$1,365

Popular ER Alpha / Estrogen Receptor Products

Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note intense nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-Estrogen Receptor antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1470 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note intense nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Bovine, Human, Mouse, Rat, Rabbit
Applications: Immunoprecipitation, Gel shift

Product Description

Estrogen Receptor antibody LS-C11577 is an unconjugated mouse monoclonal antibody to human Estrogen Receptor (ER Alpha). Validated for IHC and WB.

Specifications

Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6F11]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:40 - 1:80)
  • IHC - Frozen (1:40 - 1:60)
  • Western blot (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human estrogen receptor (alpha form).
Specificity
Recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERalpha is a ~65kD steroid hormone receptor containing an N-terminal. ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an. ERalpha binds to DNA as a homodimer and can also form heterodimers with Estrogen receptor beta The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy. Has been used successfully for identification of ERalpha on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERalpha in breast cancer tissues.
Usage
Immunohistology: Zamboni's fixative recommended for best results. #This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Presentation
Lyophilized 0.09% Sodium Azide
Reconstitution
1,000 µl Distilled Water.
Restrictions
For research use only.
About ER Alpha / Estrogen Receptor
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. P03372 NM_000125 NP_000116.2


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Requested From: United States
Date Requested: 10/22/2018

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