Human ER Alpha / Estrogen Receptor
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin (1:40 - 1:80)
- IHC - Frozen (1:40 - 1:60)
- Western blot (1:50 - 1:100)
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Recombinant human estrogen receptor (alpha form).
Recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERalpha is a ~65kD steroid hormone receptor containing an N-terminal. ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an. ERalpha binds to DNA as a homodimer and can also form heterodimers with Estrogen receptor beta The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy. Has been used successfully for identification of ERalpha on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERalpha in breast cancer tissues.
Immunohistology: Zamboni's fixative recommended for best results. #This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Lyophilized 0.09% Sodium Azide
1,000 µl Distilled Water.
For research use only.
This antibody carries the LSBio 100% Guarantee.