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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Epsin 1 / EPN1 Antibody (aa432‑481) LS‑C81966
EPN1 antibody LS-C81966 is an unconjugated rabbit polyclonal antibody to EPN1 (Epsin 1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C81966-50
50 µg
Unavailable

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Product Description

EPN1 antibody LS-C81966 is an unconjugated rabbit polyclonal antibody to EPN1 (Epsin 1) from human, mouse, rat and other species. Validated for WB.
About Epsin 1 / EPN1
Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis. Q9Y6I3 NM_013333 NP_037465.2

EPN1 Antibody, Epsin-1 Antibody, Epsin 1 Antibody, EPS-15-interacting protein 1 Antibody


Specifications

Target
Human Epsin 1 / EPN1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Guinea pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Epsin 1 / EPN1 antibody was raised against synthetic peptide located between aa432-481 of human EPN1 (Q9Y6I3, NP_037465). Percent identity by BLAST analysis: Human, Gibbon, Galago, Mouse, Rat, Elephant, Dog, Horse (100%); Monkey, Marmoset, Guinea pig (92%); Bovine, Bat (85%).
Blocking Peptide
LS-E19816 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81966. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa432-481
Specificity
Human EPN1 / Epsin-1
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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