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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EPN3 Antibody (aa401‑450) LS‑C119961
EPN3 antibody LS-C119961 is an unconjugated rabbit polyclonal antibody to human EPN3. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C119961-50
50 µl
Unavailable
LS-C119961-100
100 µl
Unavailable

Popular EPN3 Products

Formalin-fixed and paraffin-embedded human colon carcinoma with EPN3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of EPN3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

EPN3 antibody LS-C119961 is an unconjugated rabbit polyclonal antibody to human EPN3. Validated for IHC, Peptide-ELISA and WB.

Specifications

Target
Human EPN3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
EPN3 antibody was raised against synthetic peptide from human EPN3 (aa401-450).
Epitope
aa401-450
Specificity
EPN3 Antibody detects endogenous levels of total EPN3 protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from K562 cells, using EPN3 Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/19/2018

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