Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1 - 3 µg/ml)
Immunoprecipitation (5 µg/ml)
ELISA (1 - 3 µg/ml)
Specificity and Use
Ephrin B, pan antibody was raised against synthetic peptide derived from the ephrin-B sequence.
This antibody recognizes over-expressed and endogenous ephrin-B, as well as phosphorylated ephrin-B. On Western blots it identifies a band at ~50-60kD. Reactivity has been confirmed with human 293T and MCF10A lysates and chicken cell lysates. Based on amino acid sequence homology, cross-reactivity with mouse is expected.
Western Blot: 1-3 ug/ml. Immunoprecipitation: 5 ug/ml. ELISA: 1-3 ug/ml.
PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C.