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Anti-EPHA2 / EPH Receptor A2 Antibody LS-C6535

Ordering

Wt. Vol. Conc. Price
200 µg - 1 mg/ml $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG1) to Human EPHA2 / EPH Receptor A2
Human, Mouse, Rat, Bovine, Dog
ICC, Western blot, Immunoprecipitation, Functional Assay
Unconjugated

Details

Human EPHA2 / EPH Receptor A2
Mouse
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified

Applications

ICC, Western blot, Immunoprecipitation, Functional Assay

Specificity and Use

EPHA2 / EPH Receptor A2 antibody was raised against native protein isolated by purification of phosphotyrosine-containing proteins.
Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.
Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 ug of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4 ug per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.

Packaging

0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EPHA2 / EPH Receptor A2

P29317 NM_004431 NP_004422.2

EPHA2 Antibody, CTPA Antibody, EPH receptor A2 Antibody, Ephrin type-A receptor 2 Antibody, Epithelial cell kinase Antibody, ECK Antibody, Sek-2 Antibody, ARCC2 Antibody, Soluble EPHA2 variant 1 Antibody, CTPP1 Antibody

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Requested From: United States
Date Requested: 9/29/2016

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