Target
Human EPHA2 / EPH Receptor A2
Synonyms
EPHA2 | CTPA | EPH receptor A2 | Ephrin type-A receptor 2 | Epithelial cell kinase | ECK | Sek-2 | ARCC2 | Soluble EPHA2 variant 1 | CTPP1
Reactivity
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Native protein isolated by purification of phosphotyrosine-containing proteins.
Specificity
Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.
Applications
- ICC
- Western blot
- Immunoprecipitation
- Functional Assay
Usage
Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 ug of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4 ug per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.
Presentation
0.2 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EPHA2 / EPH Receptor A2