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Anti-EPHA2 / EPH Receptor A2 Antibody (aa559-976, clone 3D7) IHC-plus™ LS-B3002

Note: This antibody replaces LS-C93701

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EPHA2 / EPH Receptor A2 Antibodies

Anti-EPHA2 / EPH Receptor A2 Antibody (Extracellular Domain) LS-C36249
Mouse Monoclonal (IgG2a) to Human EPHA2 / EPH Receptor A2
Human, Mouse, Bovine, Dog
ICC, Western blot, Flow Cytometry
Unconjugated
Anti-EPHA2 / EPH Receptor A2 Antibody (aa559-976, clone 3D7) IHC-plus™ LS-B3002
Mouse Monoclonal [clone 3D7] (IgG2b,k) to Human EPHA2 / EPH Receptor A2
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-EPHA2 / EPH Receptor A2 Antibody (N-Terminus, APC) LS-C225343
Rabbit Polyclonal (IgG) to Human EPHA2 / EPH Receptor A2
Human, Mouse
IHC, Western blot, Flow Cytometry, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3D7] (IgG2b,k) to Human EPHA2 / EPH Receptor A2
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human EPHA2 / EPH Receptor A2
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [3D7]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

EPHA2 / EPH Receptor A2 antibody was raised against recombinant human EphA2 (559-976aa) purified from E. coli. NCBI Accession No.: NP_004422.
aa559-976
Immunohistochemistry: LS-B3002 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3002 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About EPHA2 / EPH Receptor A2

P29317 NM_004431 NP_004422.2

EPHA2 Antibody, CTPA Antibody, EPH receptor A2 Antibody, Ephrin type-A receptor 2 Antibody, Epithelial cell kinase Antibody, ECK Antibody, Sek-2 Antibody, ARCC2 Antibody, Soluble EPHA2 variant 1 Antibody, CTPP1 Antibody

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Immunohistochemistry

Immunohistochemistry
Anti-EPHA2 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3002 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-EPHA2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3002 concentration 5 ug/ml.

Western blot

Western blot
The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/9/2016

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