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Anti-Eomesodermin / EOMES Antibody (N-Terminus) IHC-plus™ LS-B1533

Note: This antibody replaces LS-C30107

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular Eomesodermin / EOMES Antibodies

Anti-Eomesodermin / EOMES Antibody (N-Terminus) IHC-plus™ LS-B1533
Rabbit Polyclonal (IgG) to Human Eomesodermin / EOMES
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Eomesodermin / EOMES Antibody IHC-plus™ LS-B6801
Chicken Polyclonal to Human Eomesodermin / EOMES
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Eomesodermin / EOMES Antibody LS-C149936
Sheep Polyclonal (IgG) to Human Eomesodermin / EOMES
Human
ICC, Western blot, Flow Cytometry
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human Eomesodermin / EOMES
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human Eomesodermin / EOMES
Rabbit
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Eomesodermin / EOMES antibody was raised against synthetic peptide from N-Terminus of human EOMES (O95936, NP_005433). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%); Mouse (92%); Dog, Bovine, Bat, Elephant, Horse, Pig, Guinea pig (85%).
LS-E10478 - Lyophilized - 100 µg - $145.00
N-Terminus
Human EOMES
Immunohistochemistry: LS-B1533 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1533 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Eomesodermin / EOMES

O95936 NM_005442 NP_005433.2

EOMES Antibody, Eomesodermin Antibody, Eomesodermin homolog Antibody, T box, brain, 2 Antibody, T-box brain protein 2 Antibody, T-box brain2 Antibody, T-brain-2 Antibody, TBR-2 Antibody, TBR2 Antibody

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

Immunohistochemistry

Immunohistochemistry
Anti-EOMES antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1533 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
EOMES antibody LS-B1533 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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