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Catalog Number Size Price
LS-B5402-50 50 µg (0.5 mg/ml) $460 
ENO3 / Enolase 3 Antibody - Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ENO3 / Enolase 3 Antibody - Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10 ug/ml]
ENO3 / Enolase 3 Antibody - Western blot of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.
ENO3 / Enolase 3 Antibody - ENO3 monoclonal antibody (M01), clone 5D1 Western blot of ENO3 expression in HeLa.
ENO3 / Enolase 3 Antibody - Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
ENO3 / Enolase 3 Antibody - Western blot of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody, clone 5D1. GAPDH ( 36.1 kD ) used as specificity an.
ENO3 / Enolase 3 Antibody - Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ENO3 / Enolase 3 Antibody - Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10 ug/ml]
ENO3 / Enolase 3 Antibody - Western blot of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody (M01), clone 5D1.
ENO3 / Enolase 3 Antibody - ENO3 monoclonal antibody (M01), clone 5D1 Western blot of ENO3 expression in HeLa.
ENO3 / Enolase 3 Antibody - Detection limit for recombinant GST tagged ENO3 is approximately 0.03 ng/ml as a capture antibody.
ENO3 / Enolase 3 Antibody - Western blot of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody, clone 5D1. GAPDH ( 36.1 kD ) used as specificity an.
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IHC‑plus™ Monoclonal Mouse anti‑Human ENO3 / Enolase 3 Antibody (clone 5D1, IHC, IF, WB) LS‑B5402

IHC‑plus™ Monoclonal Mouse anti‑Human ENO3 / Enolase 3 Antibody (clone 5D1, IHC, IF, WB) LS‑B5402

Note: This antibody replaces LS-C133171
Antibody:
ENO3 / Enolase 3 Mouse anti-Human Monoclonal (5D1) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B5402-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ENO3 / Enolase 3 Mouse anti-Human Monoclonal (5D1) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Enolase 3 antibody LS-B5402 is an unconjugated mouse monoclonal antibody to human Enolase 3 (ENO3). Validated for ELISA, IF, IHC, RNAi and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ENO3 / Enolase 3
Synonyms
ENO3 | Beta-enolase | Enolase 3 | Enolase 3 (beta, muscle) | Enolase 3, (beta, muscle) | Muscle-specific enolase | MSE | Skeletal muscle enolase | GSD13
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
5D1
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
ENO3 (NP_001967, 228 aa ~ 278 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human ENO3
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ENO3 / Enolase 3
P13929 NM_001976 NP_001967.3

Validation

ENO3 / Enolase 3 Antibody - Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ENO3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ENO3 / Enolase 3 Antibody (clone 5D1) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B5402 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024