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Anti-ENO1 / Alpha Enolase Antibody (clone 8G8) IHC-plus™ LS-B4318

Note: This antibody replaces LS-C104943
Catalog Size Price
LS-B4318-50 50 µg (0.5 mg/ml) Unavailable

Most Popular ENO1 / Alpha Enolase Antibodies

Anti-ENO1 / Alpha Enolase Antibody LS-C27288
Rabbit Polyclonal (IgG) to Human ENO1 / Alpha Enolase
Human
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Unconjugated
Staining of Jurkat cells with Rabbit anti-Human NNE polyclonal antibody (LS-C27288).
Anti-ENO1 / Alpha Enolase Antibody (N-Terminus) LS-C31037
Rabbit Polyclonal (IgG) to Human ENO1 / Alpha Enolase
Human, Monkey, Bovine, Guinea pig, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ENO1 / Alpha Enolase Antibody (clone 8G8) IHC-plus™ LS-B4318
Mouse Monoclonal [clone 8G8] (IgG1,k) to Human ENO1 / Alpha Enolase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 / Alpha Enolase Antibody (aa44-93) LS-C135049
Rabbit Polyclonal to Rat ENO1 / Alpha Enolase
Rat, Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
ENO1 antibody LS-C135049 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ENO1 / Alpha Enolase Antibody (aa199-434) IHC-plus™ LS-B10960
Rabbit Polyclonal to Human ENO1 / Alpha Enolase
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Anti-ENO1 / Alpha Enolase antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10960 dilution 1:100.

100% Guaranteed
Mouse Monoclonal [clone 8G8] (IgG1,k) to Human ENO1 / Alpha Enolase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human ENO1 / Alpha Enolase
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8G8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

ENO1 (AAH22545, 1 a.a. ~ 435 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About ENO1 / Alpha Enolase

P06733 NM_001428 NP_001419.1

ENO1 Antibody, Alpha Enolase Antibody, Alpha enolase like 1 Antibody, ENO1L1 Antibody, Enolase 1 Antibody, MBP-1 Antibody, MBPB1 Antibody, MPB1 Antibody, MYC promoter-binding protein 1 Antibody, NNE Antibody, Phosphopyruvate hydratase Antibody, Alpha-enolase Antibody, Tau-crystallin Antibody, MBP1 Antibody, MPB-1 Antibody, C-myc promoter-binding protein Antibody, Enolase 1, (alpha) Antibody, Enolase-alpha Antibody, Non-neural enolase Antibody, Plasminogen-binding protein Antibody, PPH Antibody

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor.

Immunohistochemistry

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.

Western blot

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 4/21/2018

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