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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ENO1 / Alpha Enolase Antibody (clone 8G8) IHC‑plus™ LS‑B4318
Note: This antibody replaces LS-C104943
Alpha Enolase antibody LS-B4318 is an unconjugated mouse monoclonal antibody to human Alpha Enolase (ENO1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4318-50
50 µg (0.5 mg/ml)
Unavailable

Popular ENO1 / Alpha Enolase Products

Staining of Jurkat cells with Rabbit anti-Human NNE polyclonal antibody (LS-C27288).
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Guinea pig, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
ENO1 antibody LS-C135049 Western blot of Rat Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
Anti-ENO1 / Alpha Enolase antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10960 dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Western blot

Product Description

Alpha Enolase antibody LS-B4318 is an unconjugated mouse monoclonal antibody to human Alpha Enolase (ENO1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ENO1 / Alpha Enolase
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. P06733 NM_001428 NP_001419.1

ENO1 Antibody, Alpha Enolase Antibody, Alpha enolase like 1 Antibody, ENO1L1 Antibody, Enolase 1 Antibody, MBP-1 Antibody, MBPB1 Antibody, MPB1 Antibody, MYC promoter-binding protein 1 Antibody, NNE Antibody, Phosphopyruvate hydratase Antibody, Alpha-enolase Antibody, Tau-crystallin Antibody, MBP1 Antibody, MPB-1 Antibody, C-myc promoter-binding protein Antibody, Enolase 1, (alpha) Antibody, Enolase-alpha Antibody, Non-neural enolase Antibody, Plasminogen-binding protein Antibody, PPH Antibody


Specifications

Target
Human ENO1 / Alpha Enolase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [8G8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ENO1 (AAH22545, 1 a.a. ~ 435 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control MCF-7. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Presentation
PBS, pH 7.4. Sourced in Ascites.
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.

Western blot

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.

Western blot

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.

Western blot

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunohistochemistry

Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.
Anti-ENO1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4318 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.
ENO1 monoclonal antibody clone 8G8 Western blot of ENO1 expression in MCF-7.

Western blot

Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.
Western blot of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody clone 8G8.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ENO1 is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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