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Anti-ENO1 / Alpha Enolase Antibody (clone 253) LS-C204514

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ENO1 / Alpha Enolase Antibodies

Anti-ENO1 / Alpha Enolase Antibody LS-C27288
Rabbit Polyclonal (IgG) to Human ENO1 / Alpha Enolase
Human
IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
Unconjugated
Immunocytochemistry Image
Anti-ENO1 / Alpha Enolase Antibody (clone 8G8) IHC-plus™ LS-B4318
Mouse Monoclonal [clone 8G8] (IgG1,k) to Human ENO1 / Alpha Enolase
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ENO1 / Alpha Enolase Antibody (aa199-434) IHC-plus™ LS-B10960
Rabbit Polyclonal to Human ENO1 / Alpha Enolase
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 253] (IgG1,k) to Human ENO1 / Alpha Enolase
Human, Mouse, Rat, Bovine, Xenopus
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human ENO1 / Alpha Enolase
Mouse
Human, Mouse, Rat, Bovine, Xenopus (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [253]
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (1:2000 - 1:5000)
  • Immunofluorescence (1:2000 - 1:5000)
  • Western blot (1:5000 - 1:10000)

Specificity and Use

ENO1 / Alpha Enolase antibody was raised against n-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system.
Clone 253 is known to react with enolase 1 from human, bovine, mouse, rat and Xenopus. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.
Try at dilutions of 1:2000-1:5000 for immunofluorescence. For western blots try at 1:5000-1:10000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.

Packaging

PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About ENO1 / Alpha Enolase

P06733 NM_001428 NP_001419.1

ENO1 Antibody, Alpha Enolase Antibody, Alpha enolase like 1 Antibody, ENO1L1 Antibody, Enolase 1 Antibody, MBP-1 Antibody, MBPB1 Antibody, MPB1 Antibody, MYC promoter-binding protein 1 Antibody, NNE Antibody, Phosphopyruvate hydratase Antibody, Alpha-enolase Antibody, Tau-crystallin Antibody, MBP1 Antibody, MPB-1 Antibody, C-myc promoter-binding protein Antibody, Enolase 1, (alpha) Antibody, Enolase-alpha Antibody, Non-neural enolase Antibody, Plasminogen-binding protein Antibody, PPH Antibody

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor.

Immunocytochemistry

Immunocytochemistry
Rat 3T3 cells stained with ENO1 / Alpha Enolase antibody (green) and counterstained with chicken polyclonal antibody to vimentin (red) and DNA (blue). The ENO1 / Alpha Enolase antibody antibody reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments.

Western blot

Western blot
Blot of HeLa cell lysates probed with monoclonal antibody ENO1 / Alpha Enolase antibody. The antibody stains a single sharp band corresponding to enolase-1/alpha-enolase/NNE at about 47 kDa.

Requested From: United States
Date Requested: 12/8/2016

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