Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 253] (IgG1,k) to Human ENO1 / Alpha Enolase
Human, Mouse, Rat, Bovine, Xenopus
ICC, Immunofluorescence, Western blot
Human ENO1 / Alpha Enolase
Human, Mouse, Rat, Bovine, Xenopus (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal 
ICC (1:2000 - 1:5000)
Immunofluorescence (1:2000 - 1:5000)
Western blot (1:5000 - 1:10000)
Specificity and Use
ENO1 / Alpha Enolase antibody was raised against n-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system.
Clone 253 is known to react with enolase 1 from human, bovine, mouse, rat and Xenopus. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.
Try at dilutions of 1:2000-1:5000 for immunofluorescence. For western blots try at 1:5000-1:10000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.
PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor.
Rat 3T3 cells stained with ENO1 / Alpha Enolase antibody (green) and counterstained with chicken polyclonal antibody to vimentin (red) and DNA (blue). The ENO1 / Alpha Enolase antibody antibody reveals strong cytoplasmic staining, while the vimentin antibody reveals cytoplasmic intermediate filaments.
Blot of HeLa cell lysates probed with monoclonal antibody ENO1 / Alpha Enolase antibody. The antibody stains a single sharp band corresponding to enolase-1/alpha-enolase/NNE at about 47 kDa.
Requested From: United States
Date Requested: 10/21/2016