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Anti-Endothelium Antibody (clone EN4) IHC-plus™ LS-B5561

Note: This antibody replaces LS-C134844


Wt. Vol. Conc. Price
- 250 µl - Unavailable

Related Products

Most Popular Endothelium Antibodies

Anti-Endothelium Antibody (clone PAL-E) LS-C27197
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Anti-Endothelium Antibody (clone OX-43) LS-C58050
Mouse Monoclonal [clone OX-43] (IgG1) to Rat Endothelium
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Anti-Endothelium Antibody LS-C123447
Mouse Monoclonal (IgM) to Human Endothelium
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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone EN4] (IgG1) to Human Endothelium
IHC - Paraffin, IHC - Frozen, Flow Cytometry


Human Endothelium
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [EN4]
Protein G purified


  • IHC - Paraffin (1:20 - 1:100)
  • IHC - Frozen
  • Flow Cytometry

Specificity and Use

Endothelium antibody was raised against endothelium.
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
The antibody is useful for immunohistological staining of endothelia in frozen sections. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained. The antibody works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/ paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.


PBS, 0.02% sodium azide, 0.1% BSA. Sourced in TCS.
Store at 4°C.
For research use only.

About Endothelium


Anti-RECA-1 antibody IHC of human placenta, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5561 dilution 1:20.

Requested From: 
Date Requested: 3/28/2017

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