Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone EN4] (IgG1) to Human Endothelium
IHC - Paraffin, IHC - Frozen, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [EN4]
Protein G purified
IHC - Paraffin (1:20 - 1:100)
IHC - Frozen
Specificity and Use
Endothelium antibody was raised against endothelium.
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
The antibody is useful for immunohistological staining of endothelia in frozen sections. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained. The antibody works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/ paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.
PBS, 0.02% sodium azide, 0.1% BSA. Sourced in TCS.