Human, Cat, Pig
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Frozen
- Flow Cytometry
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Endothelium antibody was raised against endothelium.
Recognized the human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. Strongly stains murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells, recognizes the 130kD CD31 antigen. No other structures in the skin, heart, kidney, tonsils or spleen are stained. Species cross-reactivity: Guinea-pig, feline. Does not react with rabbit, bovine, sheep, dog, rat or mice endothelial cells.
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Immunohistochemistry: 1:20 works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.
PBS, 0.1% BSA, 0.02% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.