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Anti-Endothelium Antibody (clone EN4) LS-C123449

Catalog Size Price
LS-C123449-1000 1000 µl Unavailable

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Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone EN4] (IgG1) to Human Endothelium
Human, Cat, Pig
IHC - Frozen, ICC, Flow Cytometry
Unconjugated

Details

Human Endothelium
Mouse
Human, Cat, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [EN4]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Frozen
  • ICC
  • Flow Cytometry

Specificity and Use

Endothelium antibody was raised against endothelium.
Recognized the human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. Strongly stains murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells, recognizes the 130kD CD31 antigen. No other structures in the skin, heart, kidney, tonsils or spleen are stained. Species cross-reactivity: Guinea-pig, feline. Does not react with rabbit, bovine, sheep, dog, rat or mice endothelial cells.
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Immunohistochemistry: 1:20 works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.

Packaging

PBS, 0.1% BSA, 0.02% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.
For research use only.

About Endothelium


Requested From: 
Date Requested: 10/24/2017

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