Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Frozen (1:400)
Specificity and Use
The antigen and epitope have not been further characterized. Antigen distribution: Isolated cells: The antigen is found in variable numbers on freshly isolated umbilical vein endothelial cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercially available endothelial cell media apparently have no influence on the antigen expression. Specificity: Human: continuous endothelial cells. Other: negative in mouse negative, other species not tested.
Lyophilized from PBS pH 7.2, 10mg/ml BSA, 0.01% thimerosal