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Catalog Number Size Price
LS-C358951-30 30 µl (1 mg/ml) $255 
LS-C358951-100 100 µl (1 mg/ml) $299 
LS-C358951-200 200 µl (1 mg/ml) $351 
Endostatin Antibody - Immunohistochemical analysis of Collagen 18 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Endostatin Antibody - Immunofluorescent analysis of Collagen 18 alpha 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Endostatin Antibody - Western blot analysis of Collagen 18 alpha 1 expression in MCF7 (A) whole cell lysates.
Endostatin Antibody - Immunohistochemical analysis of Collagen 18 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Endostatin Antibody - Immunofluorescent analysis of Collagen 18 alpha 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Endostatin Antibody - Western blot analysis of Collagen 18 alpha 1 expression in MCF7 (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human Endostatin Antibody (Internal, IHC, IF, WB) LS‑C358951

Polyclonal Rabbit anti‑Human Endostatin Antibody (Internal, IHC, IF, WB) LS‑C358951

Antibody:
Endostatin Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C358951-30
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Endostatin Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Endostatin antibody LS-C358951 is an unconjugated rabbit polyclonal antibody to Endostatin (Internal) from human. It is reactive with human, bovine and chicken. Validated for ICC, IF, IHC and WB.
Target
Human Endostatin
Synonyms
COL18A1 | Collagen, type XVIII, alpha 1 | Collagen alpha-1(XVIII) chain | Endostatin | KNO | KNO1 | KS | Multi-functional protein MFP | Antiangiogenic agent | Collagen alpha 1(xviii) | Knobloch syndrome, type 1
Host
Rabbit
Reactivity
Human, Bovine, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Collagen 18 alpha 1.
Epitope
Internal
Specificity
Recognizes endogenous levels of Collagen 18 alpha 1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Endostatin
P39060 NM_030582 NP_085059.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024