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Anti-Endostatin Antibody IHC-plus™ LS-B2229

Note: This antibody replaces LS-C23045
Catalog Size Price
LS-B2229-50 50 µl Unavailable

Related Products

67 Endostatin Antibodies

Most Popular Endostatin Antibodies

Anti-Endostatin Antibody (C-Terminus) IHC-plus™ LS-B2163
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Immunohistochemistry Image
Anti-Endostatin Antibody IHC-plus™ LS-B2229
Mouse Monoclonal (IgG) to Mouse Endostatin
Mouse, Human, Rat
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Immunohistochemistry Image
Anti-Endostatin Antibody (C-Terminus) LS-C65320
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Anti-Endostatin Antibody (aa801-850) IHC-plus™ LS-B5686
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Anti-Endostatin Antibody IHC-plus™ LS-B8215
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100% Guaranteed
Mouse Monoclonal (IgG) to Mouse Endostatin
Mouse, Human, Rat
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Mouse Endostatin
Mouse
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Thiophilic adsorption
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

Endostatin antibody was raised against full-length recombinant murine endostatin.
Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
Immunohistochemistry: LS-B2229 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2229 was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.

Packaging

50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Endostatin

P39060 NM_030582 NP_085059.2

COL18A1 Antibody, Collagen, type XVIII, alpha 1 Antibody, Collagen alpha-1(XVIII) chain Antibody, Endostatin Antibody, KNO Antibody, KNO1 Antibody, KS Antibody, Multi-functional protein MFP Antibody, Antiangiogenic agent Antibody, Collagen alpha 1(xviii) Antibody, Knobloch syndrome, type 1 Antibody

Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors.

Immunohistochemistry

Immunohistochemistry
Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.

Requested From: 
Date Requested: 12/12/2017

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