Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (1:100)
- Western blot
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Endostatin antibody was raised against full-length recombinant murine endostatin.
Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
Immunohistochemistry: LS-B2229 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2229 was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.