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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Endostatin Antibody IHC‑plus™ LS‑B2229
Note: This antibody replaces LS-C23045
Endostatin antibody LS-B2229 is an unconjugated mouse monoclonal antibody to Endostatin from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2229-50
50 µl
Unavailable

Popular Endostatin Products

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Product Description

Endostatin antibody LS-B2229 is an unconjugated mouse monoclonal antibody to Endostatin from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About Endostatin
Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. P39060 NM_030582 NP_085059.2

COL18A1 Antibody, Collagen, type XVIII, alpha 1 Antibody, Collagen alpha-1(XVIII) chain Antibody, Endostatin Antibody, KNO Antibody, KNO1 Antibody, KS Antibody, Multi-functional protein MFP Antibody, Antiangiogenic agent Antibody, Collagen alpha 1(xviii) Antibody, Knobloch syndrome, type 1 Antibody


Specifications

Target
Mouse Endostatin
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Thiophilic adsorption
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Endostatin antibody was raised against full-length recombinant murine endostatin.
Specificity
Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
Usage
Immunohistochemistry: LS-B2229 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2229 was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Presentation
50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.
Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.

Immunohistochemistry

Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.
Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.

Immunohistochemistry

Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.
Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.

Immunohistochemistry

Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.
Anti-COL18A1 / Endostatin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2229 dilution 1:100.


Requested From: 
Date Requested: 6/19/2018

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