Human EN2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with FITC, Biotin, HRP.

Immunoaffinity purified

Unmodified

Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%); Bat, Opossum, Zebra finch, Chicken, Platypus (92%); Xenopus, Zebrafish (84%).

C-Terminus

Human EN2

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.05 µg/ml)

Immunohistochemistry: LS-B3477 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3477 was determined to be 5 ug/ml.

PBS, 0.09% sodium azide, 2% sucrose

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P19622 NCBI: NM_001427 NP_001418.2