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EMG1 / NEP1

EMG1 N1-specific pseudouridine methyltransferase

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.

Gene Name: EMG1 N1-specific pseudouridine methyltransferase
Synonyms: EMG1, C2F, BWCNS, Grcc2f, Essential for mitotic growth 1, NEP1, Protein C2f, Nucleolar protein EMG1 homolog
Target Sequences: NM_006331 NP_006322.4 Q92979

☰ Filters
Antibodies (20)
Proteins (4)
Over-Expression Lysate (2)
Primary (20)
Recombinant (2)
EMG1 / NEP1 (24)
Human (19)
Mouse (4)
Rat (4)
Chimpanzee (3)
Dog (1)
Gibbon (3)
Guinea pig (3)
Hamster (3)
Horse (3)
Monkey (4)
Orangutan (3)
Rabbit (3)
IHC (4)
IHC-P (4)
WB (20)
Flo (9)
ELISA (7)
IF (4)
rabbit (17)
mouse (3)
IgG (13)
IgG2b (2)
monoclonal mc (2)
polyclonal pc (18)
OTI1B8 (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
Carrier-free (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (9)
aa104-133 (7)
C-Terminus (4)
aa51-100 (4)
No (24)
His (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
EMG1 / NEP1 Antibody - Western blot analysis of extracts of various cells.
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EMG1 / NEP1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
EMG1 / NEP1 Antibody - Anti-EMG1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.
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EMG1 / NEP1 Mouse anti-Human Monoclonal (OTI1B8) Antibody
Flo, IF, WB
Unconjugated
100 µl/$434
EMG1 / NEP1 Antibody - Anti-EMG1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.
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EMG1 / NEP1 Mouse anti-Human Monoclonal (Carrier-free) (OTI1B8) Antibody
Dog, Human, Monkey
Flo, IF, WB
Unconjugated, Carrier-free
100 µg/$511
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$331
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) Antibody
Human
ELISA, Flo, WB
Unconjugated
200 µl/$734
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) (Biotin) Antibody
Human
ELISA, Flo, WB
Biotin Conjugated
200 µl/$952
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) (FITC) Antibody
Human
ELISA, Flo, WB
FITC Conjugated
200 µl/$952
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) (APC) Antibody
Human
ELISA, Flo, WB
APC Conjugated
200 µl/$952
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) (PE) Antibody
Human
ELISA, Flo, WB
PE Conjugated
200 µl/$952
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, WB
HRP Conjugated, Azide-free
200 µl/$952
EMG1 / NEP1 Antibody - EMG1 antibody ARP40636_P050-NP_006322-EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa51-100) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$424
EMG1 / NEP1 Antibody - EMG1 antibody ARP40636_P050-NP_006322-EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa51-100) (HRP) Antibody
Human
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
EMG1 / NEP1 Antibody - EMG1 antibody ARP40636_P050-NP_006322-EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa51-100) (Biotin) Antibody
Human
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
EMG1 / NEP1 Antibody - EMG1 antibody ARP40636_P050-NP_006322-EMG1 (EMG1 nucleolar protein homolog (S. cerevisiae)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa51-100) (FITC) Antibody
Human
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
EMG1 / NEP1 Antibody - EMG1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The EMG1 antibody detected the EMG1 protein (arrow).
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EMG1 / NEP1 Rabbit anti-Human Polyclonal (aa104-133) Antibody
Human
Flo, WB
Unconjugated
400 µl/$393
Select
EMG1 / NEP1 Mouse anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
50 µg/$705
EMG1 / NEP1 Antibody - Western blot of Human THP-1. EMG1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
EMG1 / NEP1 Antibody - Western blot of Human THP-1. EMG1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody
Rabbit, Mouse, Guinea pig, Rat, Hamster, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100 µl/$469
EMG1 / NEP1 Antibody - Western blot of Human THP-1. EMG1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Rabbit, Mouse, Guinea pig, Rat, Hamster, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
EMG1 / NEP1 Antibody - Western blot of Human THP-1. EMG1 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
EMG1 / NEP1 Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody
Rabbit, Mouse, Guinea pig, Rat, Hamster, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
EMG1 / NEP1 Protein - Purified recombinant protein EMG1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
26.5 kDa
20 µg/$1,107
EMG1 / NEP1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
26.5 kDa
20 µg/$150
EMG1 / NEP1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
26.5 kDa
100 µg/$494
EMG1 / NEP1 Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Viewing 1-24 of 24 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).