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Anti-EMG1 / NEP1 Antibody (clone 1B8) LS-C115237

Ordering

Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular EMG1 / NEP1 Antibodies

Anti-EMG1 / NEP1 Antibody (aa111-140, AP) LS-C208879
Rabbit Polyclonal (IgG) to Human EMG1 / NEP1
Human
Western blot, Flow Cytometry, ELISA
AP Conjugated
Anti-EMG1 / NEP1 Antibody (aa111-140, APC) LS-C208880
Rabbit Polyclonal (IgG) to Human EMG1 / NEP1
Human
Western blot, Flow Cytometry, ELISA
APC Conjugated
Anti-EMG1 / NEP1 Antibody (aa111-140, Azide-free, HRP) LS-C208883
Rabbit Polyclonal (IgG) to Human EMG1 / NEP1
Human
Western blot, Flow Cytometry, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1B8] (IgG2b) to Human EMG1 / NEP1
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human EMG1 / NEP1
Mouse
IgG2b Monoclonal [1B8]
Unconjugated
Affinity purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

EMG1 / NEP1 antibody was raised against full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell
Human EMG1
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About EMG1 / NEP1

Q92979 NM_006331 NP_006322.4

EMG1 Antibody, C2F Antibody, BWCNS Antibody, Grcc2f Antibody, Essential for mitotic growth 1 Antibody, NEP1 Antibody, Protein C2f Antibody, Nucleolar protein EMG1 homolog Antibody

S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967).

Immunofluorescence

Immunofluorescence
Anti-EMG1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EMG1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EMG1.

Western blot

Western blot
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EMG1 monoclonal antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometric Analysis of Hela cells, using anti-EMG1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
 

Requested From: United States
Date Requested: 12/4/2016

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