MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Ammonium sulfate precipitation
EMA / MUC1 antibody was raised against purified human Epithelial Membrane Antigen.
Recognizes Human surface glycoprotein of approximately 54kD. Positive against all current carcinoma cell lines.
Suitable for use in Immunohistochemistry. Immunohistochemistry: 1:25-1:50 (streptavidin: biotin). Formalin-fixed, paraffin-embedded tissue sections can be stained successfully but require heat treatment to restore antigenicity, before staining. This involves exposure of tissue sections to boiling 0.01 M citrate buffer, pH 6.0 for 25-30 minutes or for 2-3 minutes at elevated pressure.
PBS, pH 7.2, 1% BSA, 0.1% sodium azide, 40%. Glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.