MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
EMA / MUC1 antibody was raised against high molecular weight (>300kD) glycoprotein from human milk-fat globule molecule. Percent identity by BLAST analysis: Human (100%); Gorilla (96%); Gibbon (92%).
Binds with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA syn pep spanning the one repeat of VNTR extracellular potion of MUC1 molecule. Reacts with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. Does not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific MAB. No cross-reaction with egg white avidin.
Suitable for use in ELISA tests for detection and measurements of unglycosylated and underglycosylated tumor-associated MUC1 mucins in human serum. Also for use in solid-phase reagents for MUC1 isoforms capture (a) in conjunction with the same monoclonal enzyme-conjugated versions (detection of unglycosylated MUC1), or (b) with enzyme-conjugated monoclonal specific to carbohydrate portions of MUC1 (detection of underglycosylated MUC1).
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.