Human EMA / MUC1

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

SPM492

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Breast cancer cell line ZR75-1.

Human EMA / MUC1

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.25 - 0.5 µg/10E6 cells)
• ELISA (2 - 5 µg/ml)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Optimal dilution of the anti-MUC-1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15941 NCBI: NM_002456 NP_002447.4