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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ELP2 / STATIP1 Antibody (aa679‑693) LS‑C10446 Best Seller
STATIP1 antibody LS-C10446 is an unconjugated rabbit polyclonal antibody to STATIP1 (ELP2) from human, mouse, rat and other species. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C10446-200
200 µg (1 mg/ml)
$620

Popular ELP2 / STATIP1 Products

Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemistry of Human thyroid cancer using ELP2 Polyclonal Antibody at dilution of 1:40.
Species: Human, Rat
Applications: IHC, ELISA
Immunohistochemistry of Human colon cancer using ELP2 Polyclonal Antibody at dilution of 1:40.
Species: Human, Rat
Applications: IHC, ELISA
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

STATIP1 antibody LS-C10446 is an unconjugated rabbit polyclonal antibody to STATIP1 (ELP2) from human, mouse, rat and other species. Validated for IP and WB.
About ELP2 / STATIP1
Regulates the ligand-dependent activation of STAT3. Q6IA86 NM_018255 NP_060725.1

ELP2 Antibody, Elongation protein 2 homolog Antibody, Elongator complex protein 2 Antibody, Elongator protein 2 Antibody, SHINC-2 Antibody, STATIP1 Antibody, STIP Antibody, STAT3-interacting protein 1 Antibody


Specifications

Target
Mouse ELP2 / STATIP1
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
ELP2 / STATIP1 antibody was raised against synthetic peptide aa 679-693 (CSPDSKYFFTGSRDKK) corresponding to murine StIP-1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Panda, Dog, Rabbit (100%); Mouse, Bat, Horse, Opossum (93%); Elephant, Bovine, Chicken, Xenopus, Stickleback, Pufferfish (87%); Blood fluke (80%).
Epitope
aa679-693
Specificity
Recognizes StIP-1 at 92kD, which may appear as a doublet. Also recognizes a second band at ~70kD. Species cross-reactivity: Mouse. Species sequence homology: human- highly conserved.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 1-4 ug immunoprecipitated StIP-1 from 500 ug of BAF-3 RIPA cell lysate. Reported to immunoprecipitate human StIP-1.
Presentation
0.07 M Tris-glycine, pH 7.4, 0.105 M sodium chloride, 0.035% sodium azide, 30% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/20/2018

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