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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ELMO3 Antibody (aa398‑447) LS‑C80519
ELMO3 antibody LS-C80519 is an unconjugated rabbit polyclonal antibody to ELMO3 from human, mouse, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C80519-50
50 µg
Unavailable

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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

ELMO3 antibody LS-C80519 is an unconjugated rabbit polyclonal antibody to ELMO3 from human, mouse, bovine and other species. Validated for WB.
About ELMO3
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Q96BJ8 NM_024712 NP_078988.2

ELMO3 Antibody, Ced-12 homolog 3 Antibody, ELMO-3 Antibody, Engulfment and cell motility 3 Antibody, CED-12 Antibody, CED12 Antibody


Specifications

Target
Human ELMO3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Predicted
Rat, Bat, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
ELMO3 antibody was raised against synthetic peptide located between aa398-447 of human ELMO3 (Q96BJ8, NP_078988). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Dog, Bovine, Pig, Opossum, Guinea pig, Platypus (100%); Rat, Bat, Rabbit, Horse (92%).
Blocking Peptide
LS-E15699 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80519. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa398-447
Specificity
Human ELMO3
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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